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Protein degradation | Topic profile

Topic profile page for Protein degradation. This page has aggregated data from forum posts, threads, listings, online discussions, newsgroups, messageboards, and other online sources which contain user generated content for the term: Protein degradation.
Topic "Protein degradation" was discussed 98 times on 37 sites in last 3 months
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Most Recent Posts protein degradation after purification  Biocompare Forums - site profile Most Recent Posts - forum profile  Go to this thread  protein degradation after purification
Protein Forums protein degradation  Biocompare Forums - site profile Protein Forums - forum profile  Go to this thread  protein degradation
Supplement Science EPA and Protein degradation.  Bodybuilding.com Forums - Bodybuilding And Fitness Board - site profile Supplement Science - forum profile  Go to this thread  EPA and Protein degradation.
Biochemistry,Genetics, Molecular biology Protein Degradation: The Ubiquitin-Proteasome System and...  ط¨ط±ط§ظ…ط¬ ظ†طھ - site profile Biochemistry,Genetics, Molecular biology - forum profile  Go to this thread  Protein Degradation: The Ubiquitin-Proteasome System and Disease

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Started 3 days, 1 hour ago (2009-11-27 22:32:00)  by nisse
hi! i've realized that my his-strep-tagged protein gets degraded after purification during storage at -20°C or 4°C... im using EDTA-free tabs during lysis. it's then dialysed against PBS pH7.4. do you have any idea what i could do? i.e. what buffer i could try to use? thanks a lot for you help!!!
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Basic Lab Protocols and Techniques
Started 3 weeks, 5 days ago (2009-11-04 17:46:00)  by oracle6781
Hello everyone, I was just wondering how long samples can be kept frozen at -80C before degradation? They are in 100 ul lysis buffer with 1mM leupeptin and and 1mM aprotinin. Thanks for your help.
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Basic Lab Protocols and Techniques
Started 3 weeks, 4 days ago (2009-11-05 16:23:00)  by danfive
Depends on the nature of the sample, serum/plasma/tissue etc. You can try a protease inhibitor cocktail and sodium orthovanadate, and DTT if it is appropriate for your downstream applications.
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Supplements
Started 2 months, 1 week ago (2009-09-22 04:44:00)  by elementrip
Protein: Whey protein has high surface area. Oxygen, & water vapor slowly diffuse through the plastic packaging naturally, you simply sped this process up. There is definitely some chemical degradation of the proteins, and their amino acids. You can get denaturation, cross linking, and other reactions. The packaging it was kept in plays a very key role in this. I wouldn't necessarily assume...
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