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Thread: using blast
Started 2 months, 2 weeks ago by blue_bear
Hi I am trying to find some orthologs and paralogs for this gene called san-1 in C. elegans with blast. I'm not quite sure if i'm using the blast correctly as all I seem to find is one ortholog called Bub-1 ?? any hints to using blast to find homologs? thanks in advance!
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Forum: Proteomics Forum   Proteomics Forum - forum profile
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blue_bear replied 2 months, 2 weeks ago
what is the difference between the %identity and %positive? How do I interpret these % for them to be significant homologs? and what about e value. I know an E value of <0.001 is significant.
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using blast
Published (2009-10-17 22:41:00)
what is the difference between the %identity and %positive? How do I interpret these % for them to be significant homologs? and what about e value. I know an E value of &lt;0.001 is significant.

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