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Thread: {help} RNA degrade
Started 2 months, 3 weeks ago by tomoe
RNA was extracted about 10 months ago. I kept them at -80 C freezer. now, i do my realtime PCR using those RNA. I confuse, the Ct value >35 cycle my RNA was degrade? how could we check?
Site: www.molecularstation.com  www.molecularstation.com - site profile
Forum: RNA Techniques Forum   RNA Techniques Forum - forum profile
Total authors: 2 authors
Total thread posts: 3 posts
Thread activity: no new posts during last week
Domain info for: molecularstation.com

Other posts in this thread:

danfive replied 2 months, 3 weeks ago
check the RNA quality on acrylamide gel.
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danfive replied 2 months, 3 weeks ago
You can also try to amplify a housekeeping gene/transcript.
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Top contributing authors

Name
Posts
danfive
2
user's latest post:
{help} RNA degrade
Published (2009-09-28 18:22:00)
You can also try to amplify a housekeeping gene/transcript.
tomoe
1
user's latest post:
{help} RNA degrade
Published (2009-09-25 07:48:00)
RNA was extracted about 10 months ago. I kept them at -80 C freezer. now, i do my realtime PCR using those RNA. I confuse, the Ct value &gt;35 cycle my RNA was degrade? how could we check?

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