Started 1 week ago (2009-11-18 10:18:00)
by Aga
High efficiency may be caused by
amplification of unspecific products together with specific ones. Check for primer-dimers, specificity of your primers and possible contamination problems.
Started 1 week, 1 day ago (2009-11-17 14:25:00)
by danfive
Quote:
Originally Posted by sagarp what are the scopes of biochemistry?i have heard that in the
underdeveloped countries there is not its scope,is it true? Re-phrase that question, it doesn't make sense.
Started 1 week, 1 day ago (2009-11-18 03:27:00)
by kerstengeon
wow i learned a lot thanks
Started 4 days, 10 hours ago (2009-11-21 20:27:00)
by kmunson779
Have you spun down your sample yet? If not, then just add more
ethanol until you get the final percentage you were aiming for. You may have to split your sample into a couple of tubes if the volume gets too high, but your sample should spin down fine. I also suggest adding some carrier to help it come down; we use Glycoblue.
If you have spun down your sample, didn't see a pellet but went ...
Started 9 months, 2 weeks ago (2009-02-11 17:49:00)
by Aga
Try
primers concentration first on a few known samples - preferably standards, to see which concentration is the best - if you work with SYBR Green (as you say) choose this primers concentration which will give you good amplification curve but with minimal primer dimers - this you'll see after melting curve. Make all necessary normalization reactions on a few known samples - together with ...