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www.molecularstation.com - www.molecularstation.com | Site profile
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Site profile page for http://www.molecularstation.com.
This report page has aggregated and summarized the online discussions from the Message Board located at http://www.molecularstation.com.
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2) Top 10 Site Forums
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Posting activity table on www.molecularstation.com:
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3 Months
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Rating - The position measured by activity among all forum sites tracked by BoardReader.
If rating is 10 there are 9 forum sites which have higher activity.
Posts - Number of posts on forum site during last 7 days.
Threads - Number of threads on forum site active during last 7 days.
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www.molecularstation.com posting activity graph:
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http://www.molecularstation.com Alexa graph:
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Top authors on www.molecularstation.com during last week:
user's latest post:
Looking for Cell with unique...
Published (2009-11-06 15:21:00)
There are lotsu of neat cells, especially if you look at single-celled organisms. All kinds of neat shapes and unusual features. I'd look into diatoms for inspiration - they are single celled plants that often look like little crystalline snowflakes. They may also be easier to model as they are more geometric and are usually symmetrical. A couple of links: [Only registered users see links. ] [Only registered users see links. ] Bryan PS:...
user's latest post:
siRNA gene silencing?
Published (2009-11-07 10:30:00)
And see that video near the heading of your thread no...
user's latest post:
Mycoplasma contamination?
Published (2009-11-08 14:24:00)
Probably, there are no straightforward ways to detect a mycoplasma contamination. However, for this porpuse there is the Hoechst staining solution (Sigma, cat# H6024). Unlike other methods, there's no need to perform PCR, even though you need a flourescence microscopy.
user's latest post:
Call for articles!
Published (2009-11-05 14:05:00)
I am beginning a new online publication called MHMNews. It will focus on the molecular and energetic basis of health and wellness. The theme for the first issue in January 2010 is Immunity. If anyone is interested in contributing or would like more info on this, please let me know. Henry
user's latest post:
co-transfection protocol for...
Published (2009-10-30 14:36:00)
Hi, I've posted a protocol for siRNA and DNA transfection protocol but it should work for 2 plasmids hope it will help xang
user's latest post:
Adipose tissue problem-very urgent
Published (2009-11-05 16:27:00)
Red stuff inhibiting protein assay may be heme. You can run your protein sample (clear viscous supernatant) through a Chromaspin column (T100?), take the eluant and run it through a second chromaspin column, and try the eluant in the protein assay.
user's latest post:
cell culture help
Published (2009-11-04 02:21:00)
Thanks for the ideas! The lighting was not intended for sanitizing purposes-the tech just liked the additional lighting and left them on nine hours a day-quite intense. We have gone back to our frozen lines, with similar problems. I suspect a problem in the production of our MEM. We add Sodium Bicarb and RO water to a powdered MEM and go through a filtering process. We store this and make up a 10% or 2% FBS media as needed. I am also...
user's latest post:
Looking for Cell with unique...
Published (2009-11-05 23:48:00)
"Bradley K. Sherman" < [Only registered users see links. ] > wrote in message news:hcvggu$s8i$ [Only registered users see links. ] ... Thanks Bradley, That's neat, looks so much like an insect walking on that bubble. Just so I'm clear, that is a single cell and the cilia are part of that cell. Many more on the site that I'll check out too. Thanks, The Mav
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Top 10 active forums on www.molecularstation.com during last week:
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Top 10 forums on www.molecularstation.com:
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Latest active threads on www.molecularstation.com:
Started 6 days, 12 hours ago (2009-11-03 14:28:00)
by Warthaug
Easy, there are four possible bases, meaning your binary code would be a 2-digit code. For example:
00 = A
01 = T
10 = C
11 = G
Of course, you can use any combination you like. The above system is particularity efficient, as 4 letters can be stored in one byte; normally, 1 byte (8 bits) encodes one letter.
Bryan
Started 1 day, 9 hours ago (2009-11-08 17:17:00)
by sksdam802
as u know that the replication of plasmid need energy in 50-60% the cells will get gud energy frome media so it will replicte the plasmid also and in 70% the source of enery will be less so it will not allow the plasmid to rep
Started 1 day, 9 hours ago (2009-11-08 17:11:00)
by sksdam802
no because it will be antigen for the other person
Started 1 day, 12 hours ago (2009-11-08 14:24:00)
by Winter
Probably, there are no straightforward ways to detect a mycoplasma contamination.
However, for this porpuse there is the Hoechst staining solution (Sigma, cat# H6024). Unlike other methods, there's no need to perform PCR, even though you need a flourescence microscopy.
Started 3 days, 6 hours ago (2009-11-06 19:56:00)
by Winter
Probably, the molecules that best fit your definition are the dideoxynucleotides (ddNTPs), the chain terminators used for the Sanger's DNA sequencing method.
Started 2 days ago (2009-11-08 01:49:00)
by lala0453
Started 2 weeks ago (2009-10-26 03:51:00)
by Archonster
RPMI 1640 formulation could be found in invitrogen web site.
FBS supply many many growth factor to cell growth...
Started 2 days, 16 hours ago (2009-11-07 10:19:00)
by nanofreak
First you need to know about RNAi- You can google for loads of info or see it on wikipedia
but before that see this HHMI video its very good. Once you understand RNAi you can go on to siRNA
oh I'm not able to link the video here
Ok just go to youtube and search for 'RNAi HHMI' its in 2 parts and its good
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Hot threads for last week on www.molecularstation.com:
Started 6 days, 12 hours ago (2009-11-03 14:32:00)
by Warthaug
A fluorescent light in a storage area is not an effective way of controlling bacterial growth - the intensity of the light drops off with the square of the distance, meaning unless something is close to the bulb it'll not get sanitised.
The UV could be breaking down your media, although for the above reason's I suspect that's not the issue.
Did you do microplasma testing? Your problem ...
Started 2 days, 16 hours ago (2009-11-07 10:19:00)
by nanofreak
First you need to know about RNAi- You can google for loads of info or see it on wikipedia
but before that see this HHMI video its very good. Once you understand RNAi you can go on to siRNA
oh I'm not able to link the video here
Ok just go to youtube and search for 'RNAi HHMI' its in 2 parts and its good
Started 3 days, 6 hours ago (2009-11-06 19:56:00)
by Winter
Probably, the molecules that best fit your definition are the dideoxynucleotides (ddNTPs), the chain terminators used for the Sanger's DNA sequencing method.
Started 4 days, 5 hours ago (2009-11-05 21:36:00)
by Bradley K. Sherman
In article <hcvfdo$huh$ [Only registered users see links. ] >,
amdx < [Only registered users see links. ] > wrote:
Surprisingly, each cell that exists, and has ever existed, has
unique composition.
Check out these cells:
<http://www.microscope-microscope.org/application s/pond-critters/protozoans/ciliphora/euplotes.htm>
-- bks
Started 2 weeks ago (2009-10-26 03:51:00)
by Archonster
RPMI 1640 formulation could be found in invitrogen web site.
FBS supply many many growth factor to cell growth...
Started 6 days, 12 hours ago (2009-11-03 14:28:00)
by Warthaug
Easy, there are four possible bases, meaning your binary code would be a 2-digit code. For example:
00 = A
01 = T
10 = C
11 = G
Of course, you can use any combination you like. The above system is particularity efficient, as 4 letters can be stored in one byte; normally, 1 byte (8 bits) encodes one letter.
Bryan
Started 3 months ago (2009-08-05 11:17:00)
by Zel
What is (in a nutshell) stem cell research?
Please post websites that are useful with information
Thanks!
Started 1 week, 1 day ago (2009-11-01 22:18:00)
by osama al refa'e
i need some one to tell general information about BioStatistics?
and if there any link to visit.........
thanx
Last edited by osama al refa'e; Yesterday at 10:20 PM .
Started 4 days, 10 hours ago (2009-11-05 16:23:00)
by danfive
Depends on the nature of the sample, serum/plasma/tissue etc.
You can try a protease inhibitor cocktail and sodium orthovanadate, and DTT if it is appropriate for your downstream applications.
Started 3 days, 14 hours ago (2009-11-06 12:09:00)
by nanofreak
but molecular weight is already shown in swissprot I thought, next to the FASTA sequence of the isoform or its ID number?
Oh sorry u want like a tabular list... OK...Understood.
this seems to be the reverse of what you asked, but it still might be useful to someone else [Only registered users see links. ]
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