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Biocompare Forums - www.biocompare.com | Site profile
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Site profile page for http://www.biocompare.com.
This report page has aggregated and summarized the online discussions from the Message Board located at http://www.biocompare.com.
This site profile page outlines general site statistics such as: Users Activity, Site Activity, Site Rank, and Top Authors, which are reported in either a table or graph below for a given reporting time period.
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1) Top 10 Active Forums during Last Week
2) Top 10 Site Forums
3) Latest Active Threads
4) Hot Threads for Last Week
Warning: These statistics are generated using 'best efforts' and can experience delays and reporting errors at times. Please note that such statistics do not constitute a site's popularity and/or exact posting volumes at any given reporting period.
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Posting activity table on Biocompare Forums:
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3 Months
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Threads:
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43
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126
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696
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Post:
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49
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147
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751
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Put this code anywhere on your forum page:
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Rating - The position measured by activity among all forum sites tracked by BoardReader.
If rating is 10 there are 9 forum sites which have higher activity.
Posts - Number of posts on forum site during last 7 days.
Threads - Number of threads on forum site active during last 7 days.
Authors - Number of authors which contributed to the site within last 7 days.
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Biocompare Forums posting activity graph:
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http://www.biocompare.com Alexa graph:
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Top authors on Biocompare Forums during last week:
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user's latest post:
Endopeptidase Type1/2 &...
Published (2009-11-25 17:31:00)
Hi I am looking for endopeptidase type1 & type 2 and carboxypeptidase H(E) enzymes that are essential in the conversion of proinsulin to insulin. I am unable to find these anywhere, any help would be appreciated. thanks. Some basic info below: Endopeptidase –type 1 and 2 are cleaves proinsulin. Type 1 cleaves at the C-terminal side of the Arg31 Arg32 sequence of proinsulin whereas type 2 activity cleaves on the C-terminal side of the...
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We have a 40 micron seives that we also use. The cells are always kept on ice. The instrument keeps both the sample and the sorted cells at 4 decrees Celcius. We find that lung macrophages just tend to clump more than others. The stirrer of the Facs Aria just swirls the sample around(I think this is a bad design) and does not mix it properly. The Facs Vantage on the other hand has a proper stiirer and mixes the sample better and maybe that is...
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loss of function of ni purified...
Published (2009-11-27 22:45:00)
hi there! previous members of the lab were expressing a functionally active protein with two his tags: one c-terminal and one n-terminal (pET28 novagen). i thought that one his tag would be better and constructed a vector with only one his tag and... the protein lost its activity!!! do you have any explanation how two 6xhis tags instead of 1 6x his tag can better stabilize a protein??? the protein (around 30kDa) is dissolved in PBS pH7.4...
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Top 10 active forums on Biocompare Forums during last week:
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Top 10 forums on Biocompare Forums:
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Latest active threads on Biocompare Forums:
No activity within last 3 months.
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Hot threads for last week on Biocompare Forums:
Started 1 week, 2 days ago (2009-11-19 14:27:00)
by BlueDevil
I had some terrible blockages on the Facs Aria. I was sorting macrophages, from the lung. These macrophages tend to stick together and form clumbs. We do use a 70 micron seive before sorting to take out the clumbs. These cells just aggregate again. The aspiration tubing on the Facs Aria is very narrow as compared to the Facs Vantage. When we sorted these cells on the Facs Vantage we did not ...
Started 5 days, 9 hours ago (2009-11-23 19:36:00)
by BlueDevil
hi guys!need your advise.am working on bacterial genome.i succeeded in sequencing part of my gene throgh using primers from the sequence of related organisms.now i have designed primers for inverse pcr.i did pcr after restriction enzyme digestion then self ligation but what i can see on agarose gel is a smear.anybody with advise??i think my problem is at the ligation stage since ligated and ...
Started 4 days, 11 hours ago (2009-11-24 17:44:00)
by BlueDevil
Hi everybody, I am trying to do cytospin with some primary B cells in order to stain them. In different papers they make different conditions like 400 rpm- 5 min or 300 rpm - 10min. As most of my cells were squeezed after the cytospin (DAPI could be seen as an smear on the slide and no cell left), I reduced the cytospin conditions to 1 min- 200rpm (the mininum that the machine allows), but ...
Started 3 days, 11 hours ago (2009-11-25 17:31:00)
by BlueDevil
Hi I am looking for endopeptidase type1 & type 2 and carboxypeptidase H(E) enzymes that are essential in the conversion of proinsulin to insulin. I am unable to find these anywhere, any help would be appreciated. thanks. Some basic info below: Endopeptidase –type 1 and 2 are cleaves proinsulin. Type 1 cleaves at the C-terminal side of the Arg31 Arg32 sequence of proinsulin whereas type 2 ...
Started 3 days, 14 hours ago (2009-11-25 14:15:00)
by eliana.lopes...
Hi, my protocol need 0,1% Triton in PBS for lysis buffer . What time with 0,1 Triton should solubilize most cell proteins. Eliana
Started 2 years, 5 months ago (2007-06-26 00:00:00)
by lifescientist
I have an old protocol I wrote up for doing restriction digests, here it is in case you are interested. Streamline Protocol for General Cloning of DNA Fragments The process of DNA cloning is a critical component to target gene amplification The protocol presented here offers a method for sub-cloning DNA fragments possessing different sticky ends following ...
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