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Cell Biology and Cell Culture | Forum profile
Forum profile page for Cell Biology and Cell Culture on http://www.molecularstation.com. This report page is the aggregated overview from a single forum: Cell Biology and Cell Culture , located on the Message Board at http://www.molecularstation.com. This forum profile page summarizes the general forum statistics such as: Users Activity, Forum Activity, and Top Authors, which are reported in either a table or graph below for a given reporting time period. Additional forum profile information for "Cell Biology and Cell Culture " on the Message Board at http://www.molecularstation.com is also shown in the following ways:

1) Latest Active Threads
2) Hot Threads for Last Week

Warning: These statistics are generated using 'best efforts' and can experience delays and reporting errors at times. Please note that such statistics do not constitute a forum's popularity and/or exact posting volumes at any given reporting period.
Site: www.molecularstation.com - Cell Biology and Cell Culture
www.molecularstation.com
(site profile, domain info molecularstation.com)
Title: Cell Biology and Cell Culture
Url: http://www.molecularstation.com/forum/forumdisp...
Users activity: 13 post per thread
Forum activity: 2 active threads during last week
 

Posting activity on Cell Biology and Cell Culture :

  Week Month 3 Months
Threads: 2 17 48
Post: 3 21 60
 

Cell Biology and Cell Culture Posting activity graph:

Posts by:  day  week  month 

Top authors during last week:

Name
Posts
axv56
2
user's latest post:
NK cell conditions - IL2
Published (2009-12-02 20:36:00)
IL-12 and IL-15 are both produce by mature DCs. NK and DCs are known to interact with each other resulting in reciprocal activation. IL-12 and IL-15 promotes NK proliferation and survival. So adding either one is indeed physiologically relevant.
wangleipurdue
1
user's latest post:
culture of MCF10A in filter or...
Published (2009-12-02 04:15:00)
Hello everyone, I am trying to culture MCF10A cell in filter (cell culture insert from VWR with catalog 62404-550. It is the same thing as the one from BDbiosciences with catalog 353095). I tried to put MCF10A in those inserts directly or put cells on Collagen I coated surface, however, none of them works, the cells do not grow (the parallel one in T75 grows very well with the same medium). I put this insert in wells in a 24-well-plate, but I...
  

Latest active threads on Cell Biology and Cell Culture ::

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Started 7 months ago (2009-05-05 06:51:00)  by Gabi
Hi- I have a similar problem with NK cells which I isolate from cord blood. I have been culturing them in IL-2 and IL-15 but have a high level of apoptosis in the first few days. Where you successful with your cells? I have found that there is great variability between the different cord blood units. Do you find the same with your cells?
Thread:  Show this thread (5 posts)   Thread info: NK cell conditions - IL2 Size: 403 bytes
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Started 3 weeks, 5 days ago (2009-11-08 14:24:00)  by Winter
Probably, there are no straightforward ways to detect a mycoplasma contamination. However, for this porpuse there is the Hoechst staining solution (Sigma, cat# H6024). Unlike other methods, there's no need to perform PCR, even though you need a flourescence microscopy.
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Started 4 weeks ago (2009-11-06 19:56:00)  by Winter
Probably, the molecules that best fit your definition are the dideoxynucleotides (ddNTPs), the chain terminators used for the Sanger's DNA sequencing method.
Thread:  Show this thread (4 posts)   Thread info: > compound that block DNA replication in human cell Size: 221 bytes
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Started 1 month, 1 week ago (2009-10-26 03:51:00)  by Archonster
RPMI 1640 formulation could be found in invitrogen web site. FBS supply many many growth factor to cell growth...
Thread:  Show this thread (5 posts)   Thread info: Cell Culture: Why FBS fetal bovine serum? Size: 185 bytes
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Started 1 month ago (2009-11-04 18:11:00)  by sadhana
We have a problem in HeLa cell culture. In a 12 well plate, cells are dying in the outer wells (wells at the edges of the plate) while still growing happily in the inside (central) wells. Same cells in the T75 flask are growing fine too. Wierd, isn't it? No visible bacterial contamination either. Any thoughts?
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Started 1 month ago (2009-11-03 14:32:00)  by Warthaug
A fluorescent light in a storage area is not an effective way of controlling bacterial growth - the intensity of the light drops off with the square of the distance, meaning unless something is close to the bulb it'll not get sanitised. The UV could be breaking down your media, although for the above reason's I suspect that's not the issue. Did you do microplasma testing? Your problem ...
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Hot threads for last week on Cell Biology and Cell Culture ::

Cell Biology and Cell Culture
Started 7 months ago (2009-05-05 06:51:00)  by Gabi
Hi- I have a similar problem with NK cells which I isolate from cord blood. I have been culturing them in IL-2 and IL-15 but have a high level of apoptosis in the first few days. Where you successful with your cells? I have found that there is great variability between the different cord blood units. Do you find the same with your cells?
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