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Basic Lab Protocols and Techniques | Forum profile
Forum profile page for Basic Lab Protocols and Techniques on http://www.molecularstation.com. This report page is the aggregated overview from a single forum: Basic Lab Protocols and Techniques , located on the Message Board at http://www.molecularstation.com. This forum profile page summarizes the general forum statistics such as: Users Activity, Forum Activity, and Top Authors, which are reported in either a table or graph below for a given reporting time period. Additional forum profile information for "Basic Lab Protocols and Techniques " on the Message Board at http://www.molecularstation.com is also shown in the following ways:

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Title: Basic Lab Protocols and Techniques
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Users activity: 1 post per thread
Forum activity: 1 active thread during last week
 

Posting activity on Basic Lab Protocols and Techniques :

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Threads: 1 2 8
Post: 1 2 8
 

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Posts
ashwini_ah
1
user's latest post:
DNA isolation help!!!
Published (2009-12-04 06:43:00)
hi there, i had isolated DNA from some bacteria, fungi and streptomyces by phenol-choloroform method. when i checked them on agarose gel it showed some bright band below the dyealong with the DNA band near the wells. this i suppose is protein or RNA contamination. so i repeated the isolation afresh, this time taking care that i aspirate only aqueous phase after phenol step. the gel still shows up the same bands.... i need to use this DNA for...
  

Latest active threads on Basic Lab Protocols and Techniques ::

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Started 1 month ago (2009-11-05 16:27:00)  by danfive
Red stuff inhibiting protein assay may be heme. You can run your protein sample (clear viscous supernatant) through a Chromaspin column (T100?), take the eluant and run it through a second chromaspin column, and try the eluant in the protein assay.
Thread:  Show this thread (2 posts)   Thread info: Adipose tissue problem-very urgent Size: 313 bytes
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Started 1 month ago (2009-11-05 16:23:00)  by danfive
Depends on the nature of the sample, serum/plasma/tissue etc. You can try a protease inhibitor cocktail and sodium orthovanadate, and DTT if it is appropriate for your downstream applications.
Thread:  Show this thread (2 posts)   Thread info: Protein degradation Size: 264 bytes
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Started 4 months, 3 weeks ago (2009-07-16 23:03:00)  by gulg_genetic
You mean the membrane? I do keep them in a box at -20C. The blots, I would recommend thay you label with your name and date, and keep them in a folder..
Thread:  Show this thread (4 posts)   Thread info: How do you save a Nitrocellulose WBlot? Size: 216 bytes
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